4. Use of human gametes obtained from donors for the production of human
embryonic stem cell lines.
Oocytes were aspirated from oocyte donors (n = 12) and inseminated with frozen-thawed donor (n = 2) sperm followed by culture of embryos to day 5 or 6 in sequential media. The inner cell masses of expanded blastocysts were isolated using immunosurgery and cultured for 4-11 days. Immunosurgery of 40 blastocysts resulted in the culture of 18 inner cell masses, which have produced three cell lines. One of these cell lines has been shown to stain positive for alkaline phosphatase and stage-specific embryonic antigen (SSEA)-4 and negative for SSEA-1, express telomerase activity, and produce hCG when allowed to differentiate. These findings demonstrate that the future production of human embryonic stem cell lines for thetherapeutic use is possible with the use of donated gametes. Many ethical issues were considered before the initiation of this study, and it was the goal of the investigators to ensure that both oocyte and sperm donors understood the nature and purpose of the research before their participating in the study.
Lanzendorf SE, Boyd CA, Wright DL, Muasher S, Oehninger S, Hodgen GD (2001)
Use of human gametes obtained from anonymous donors for the production of human embryonic stem cell lines. Fertil Steril 76: 132-137